Human a1-6 fucosyltransferase

Abbreviation 

hFUT8

Source

Human

Activity

Core-fucosylaltion of N-glycan

Expression

&

Purification

Commercial Available 

Optimal Condition

37 °C

7.5

100 mM MES buffer (pH 7.0), 6 mM donor, 5 mM acceptor, incubate at 37 °C for 1 h to overnight.

Temperature

pH

Metal ion:

Example condition:   

GDP-Fuc

N-glycan

Donor:

Acceptor:

Substrate Specificity

  • Calderon A.D., Liu Y., et al. Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans. Org Biomol Chem, 2016, 14, 4027.

References