Synthetic Useful Glyco-Enzymes *
Goals: The project supported by National Institute of General Medical Sciences (U01GM116263) aims to develop facile core synthesis-enzymatic extension strategy to access diverse O-glycans and O-glycopeptides. Enzymes (glycosyltransferases) validated in the synthesis of these complex carbohydrates are summarized here. Most enzymes are derived from bacteria with high expression level and activities.
N-acetyl-hexosaminyltransferase (HexNAcT, including GalNAcT and GlcNAcT)
Only robust glycosyltransferases that validated in large scale synthesis of mammalian glycans (excluding GAG) were listed. For a more complete list of glyco-enzymes, please refer:
Schmaltz R.M., Hanson S.R., and Wong C.H.. Enzymes in the Synthesis of Glycoconjugates. Chemical Reviews, 2011, 111, 4259-4307.
Guan W., Edmunds G.A., Wu. G., and Li L. Synthetically Useful Glycosyltransferases for the Access of Mammalian Glycomes. Chapter 3 in Synthetic Glycomes, edited by Wanyi Guan, Lei Li and Peng G. Wang. 2020, 46-82.