Synthetic Useful Glyco-Enzymes *

Goals: The project supported by National Institute of General Medical Sciences (U01GM116263) aims to develop facile core synthesis-enzymatic extension strategy to access diverse O-glycans and O-glycopeptides. Enzymes (glycosyltransferases) validated in the synthesis of these complex carbohydrates are summarized here. Most enzymes are derived from bacteria with high expression level and activities. 

Galactosyltransferase (GalT)
alpha1-3GalT:
alpha1-4GalT:
beta1-3GalT:
beta1-4GalT:
Fucosyltransferase (FucT)
alpha1-2FucT:
alpha1-3FucT:
alpha1-3/4FucT:
alpha1-6FucT:
N-acetyl-hexosaminyltransferase (HexNAcT, including GalNAcT and GlcNAcT)
alpha1-3GalNAcT:
beta1-3HexNAcT:
beta1-3GalNAcT:
beta1-4GalNAcT:
Sialyltransferase (SiaT)
alpha2-3SiaT:
alpha2-6SiaT:
alpha2-3/8SiaT:
alpha2-8/9SiaT:
*
Only robust glycosyltransferases that validated in large scale synthesis of mammalian glycans (excluding GAG) were listed. For a more complete list of glyco-enzymes, please refer:
  • Schmaltz R.M., Hanson S.R., and Wong C.H.. Enzymes in the Synthesis of Glycoconjugates. Chemical Reviews, 2011, 111, 4259-4307.
  • Guan W., Edmunds G.A., Wu. G., and Li L. Synthetically Useful Glycosyltransferases for the Access of Mammalian Glycomes. Chapter 3 in Synthetic Glycomes, edited by Wanyi Guan, Lei Li and Peng G. Wang. 2020, 46-82.