Galactosyltransferase from bovine milk

Abbreviation 

b4GalT

Source

Bovine

Activity

Transfer Gal from UDP-Gal to GlcNAc via a b1,4-linkage

Expression

&

Purification

Obtained from Millipore-Sigma

CAS Number 9031-68-9 

Optimal Condition

37 °C

7.5

MnCl2, MgCl2

50 mM Tris-HCl (pH 7.5), 22 mM donor, 20 mM acceptor, 20 mM MgCl2, incubate at 37 °C for 1 h to overnight.

Temperature

pH

Metal ion:

Example condition:   

UDP-Gal

GlcNAc/Glc, recoginize GlcNAc on complex glycans

3.0-15.0 units/mg protein

Donor:

Acceptor:

Activity:

Substrate Specificity

  • Bell JE, et al. The kinetic mechanism of bovine milk galactosyltransferase. The role of alpha-lactalbumin. J Biol Chem1976, 251, 3003.

References