top of page
Galactosyltransferase from bovine milk
Abbreviation
b4GalT
Source
Bovine
Activity
Transfer Gal from UDP-Gal to GlcNAc via a b1,4-linkage
Expression
&
Purification
Obtained from Millipore-Sigma
CAS Number 9031-68-9
Optimal Condition
37 °C
7.5
MnCl2, MgCl2
50 mM Tris-HCl (pH 7.5), 22 mM donor, 20 mM acceptor, 20 mM MgCl2, incubate at 37 °C for 1 h to overnight.
Temperature:
pH:
Metal ion:
Example condition:
UDP-Gal
GlcNAc/Glc, recoginize GlcNAc on complex glycans
3.0-15.0 units/mg protein
Donor:
Acceptor:
Activity:
Substrate Specificity
-
Bell JE, et al. The kinetic mechanism of bovine milk galactosyltransferase. The role of alpha-lactalbumin. J Biol Chem, 1976, 251, 3003.
References
bottom of page